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Home > SYBR qPCR Master Mix > 2x SYBR Green qPCR Master Mix (High ROX)

2x SYBR Green qPCR Master Mix (High ROX) 

Cat.No Size Price Quantity  
B21402 5 mL USD 137
B21403 25 mL USD 580
Free Overnight Delivery on all orders over 500 USD.
Tel: (832) 582-8158Email: [email protected]


Accurate Detection: High specific and sensitive quantitation even for low copy targets or a wide range of template samples
High Stability: Hot-start enzyme activation and convenient room-temperature reaction setup makes it suitable for automation and high throughput detection
Robustness and Versatility: High-efficiency, compatible with both fast and standard qPCR cycling methods
Cost Effective: Offered at much discounted price compared to market price

Product Use Citations

Price Comparison

2x SYBR Green qPCR Master Mix (High ROX) | Price Comparison


Bimake™ 2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR Green I based quantitative PCR. The mix with high concentration of ROX, prepared at 2x reaction concentration, can be directly used for robust and low-template quantitative PCR on Applied Biosystems 5700, 7000, 7300, 7700, 7900, 7900HT, 7900HT, StepOne™, or StepOnePlus™ instruments with high sensitivity, specificity and reliability.

Two premixed versions (High ROX and Low ROX) are provided for the following qPCR instruments:

High ROX Applied Biosystems 5700, 7000, 7300, 7700, 7900, 7900HT, 7900HT Fast; StepOne™, StepOne Plus™.
Low ROX Applied Biosystems 7500, 7500 Fast, ViiA™7; Stratagene MX4000™, MX3005P™, MX3000P™, QuantiStudio™ 12K Flex.


Dye-based quantitative PCR detection
Nucleic acid amplification and expression profiling
Quantitative genotyping studies
Genomics-related applications


Component Cat #: B21402 Cat #: B21403
Bimake™ 2x SYBR Green qPCR Master Mix(High ROX) 5 mL
(for 200 reactions with 50 μL/Rxn
for 500 reactions with 20 μL/Rxn
for 1000 reactions with 10 μL/Rxn)
25 mL
(for 1000 reactions with 50 μL/Rxn
for 2500 reactions with 20 μL/Rxn
for 5000 reactions with 10 μL/Rxn)
Contains hot-start DNA Polymerase, dNTPs, Mg2+, SYBR Green I dye and ROX Dye (high concentration)


Store at -20°C for up to 2 years. Repetitive freeze-thaw cycles should be avoided. It is recommended to pre-aliquot the mix into small batches for frequent usage. Product quality is guaranteed under proper storage conditions.

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User Guide | 2x SYBR Green qPCR Master Mix (High ROX)

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