Advantages
Accurate Detection: High specific and sensitive quantitation even for low copy targets or a wide range of template samples
High Stability: Hot-start enzyme activation and convenient room-temperature reaction setup makes it suitable for automation and high throughput detection
Robustness and Versatility: High-efficiency, compatible with both fast and standard qPCR cycling methods
Cost Effective: Offered at much discounted price compared to market price
Product Use Citations
Price Comparison
Description
Bimake™ 2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR Green I based quantitative PCR. The mix with high concentration of ROX, prepared at 2x reaction concentration, can be directly used for robust and low-template quantitative PCR on Applied Biosystems 5700, 7000, 7300, 7700, 7900, 7900HT, 7900HT, StepOne™, or StepOnePlus™ instruments with high sensitivity, specificity and reliability.
Two premixed versions (High ROX and Low ROX) are provided for the following qPCR instruments:
| High ROX | Applied Biosystems 5700, 7000, 7300, 7700, 7900, 7900HT, 7900HT Fast; StepOne™, StepOne Plus™. |
| Low ROX | Applied Biosystems 7500, 7500 Fast, ViiA™7; Stratagene MX4000™, MX3005P™, MX3000P™, QuantiStudio™ 12K Flex. |
Applications
Dye-based quantitative PCR detection
Nucleic acid amplification and expression profiling
Quantitative genotyping studies
Genomics-related applications
Components
| Component | Cat #: B21402 | Cat #: B21403 |
|---|
| Bimake™ 2x SYBR Green qPCR Master Mix※(High ROX) | 5 mL
(for 200 reactions with 50 μL/Rxn
for 500 reactions with 20 μL/Rxn
for 1000 reactions with 10 μL/Rxn) | 25 mL
(for 1000 reactions with 50 μL/Rxn
for 2500 reactions with 20 μL/Rxn
for 5000 reactions with 10 μL/Rxn) |
| ※ Contains hot-start DNA Polymerase, dNTPs, Mg2+, SYBR Green I dye and ROX Dye (high concentration) |
Storage
Store at -20°C for up to 2 years. Repetitive freeze-thaw cycles should be avoided. It is recommended to pre-aliquot the mix into small batches for frequent usage. Product quality is guaranteed under proper storage conditions.
File Download
User Guide | 2x SYBR Green qPCR Master Mix (High ROX)
