Advantages
Time saving: save 15-30 min comparing with agarose beads.
Simple operation: Magnetic separation and centrifugation-free.
High protein loading capacity.
High specificity.
Customer Reviews
Customer Product Validation(2)
Price Comparison
Description
Anti-Flag magnetic beads is based on hydroxyl magnetic beads covalently coupling with high quality recombinant mouse monoclonal antibody. With high loading of Flag-tagged protein (more than 0.6 mg protein/mL) and high specificity, it is recommended to use for co-immunoprecipitation and protein purification.
Properties
| Antibody isotype | Recombinant mouse monoclonal antibody |
|---|
| Antibody purification | Protein A purified |
|---|
| Application | Immunoprecipitation and protein Purification |
|---|
| Recommended volume | IP: 10 μl gel for 500 μl crude protein solution |
|---|
| Binding capacity | Minimum 0.6 mg protein eluted per ml of magnetic beads |
|---|
| Binding characteristics | Met-N-terminal FLAG fusion protein: Met-FLAG–Protein
N-terminal FLAG fusion protein: FLAG–Protein
C-terminal FLAG fusion protein: Protein-FLAG |
|---|
| Shipped in | Blue ice |
|---|
Storage
Store at 2-8°C for 2 years. DO NOT freeze or centrifuge Magnetic Beads.
Protocol
Trouble Shooting
| Problem | Possible Reason | Suggested Improvement |
|---|
| High background | Nonspecifically binding of proteins to the antibody, megnetic beads or EP tubes | Pre-clear lysate to remove nonspecific binding proteins.
After suspending beads for the final wash, transfer the entire sample to a clean EP tube and then centrifugation. |
| Washing times are not sufficient. | Increase the number of washes.
Increase duration time of washes. |
| No signal is observed. | Flag tagged protein is not expressed in the sample. | Make sure the protein of interest contains the FLAG sequence.
Prepare the fresh lysate.
Use appropriate protease inhibitors. |
| Incubation times are inadequate. | Increase the incubation times. |
| Interfering substance is present in sample. | The lysate may contain high concentrations of dithiothreitol (DTT), 2-mercaptoethanol, or other reducing agents.
Excessive detergent concentration may interfere with the antibody-antigen interaction. |
File Download
Anti-Flag magnetic beads
