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Anti-Flag magnetic beads 

Cat.No. Size Price Quantity  
B26101 1 mL USD 319
B26102 5 mL USD 999
Free Overnight Delivery on all orders over 500 USD.
Tel: (832) 582-8158Email: [email protected]

Advantages

Time saving: save 15-30 min comparing with agarose beads.
Simple operation: Magnetic separation and centrifugation-free.
High protein loading capacity.
High specificity.

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Description

Anti-Flag magnetic beads is based on hydroxyl magnetic beads covalently coupling with high quality mouse IgG1 monoclonal antibody. With high loading of Flag-tagged protein (more than 1.1 mg protein/mL) and high specificity, it is recommended to use for co-immunoprecipitation and protein purification.

Properties

Antibody isotype Mouse IgG1
Antibody purification Protein A purified
Application Immunoprecipitation and protein Purification
Recommended volume IP: 10 μl gel for 500 μl crude protein solution
Binding capacity Minimum 1.1 mg protein eluted per ml of magnetic beads
Binding characteristics Met-N-terminal FLAG fusion protein: Met-FLAG–Protein
N-terminal FLAG fusion protein: FLAG–Protein
C-terminal FLAG fusion protein: Protein-FLAG
Shipped in Blue ice

Storage

Store at 2-8°C for 2 years. DO NOT freeze or centrifuge Magnetic Beads.

Protocol

Trouble Shooting

Problem Possible Reason Suggested Improvement
High background Nonspecifically binding of proteins to the antibody, megnetic beads or EP tubes Pre-clear lysate to remove nonspecific binding proteins.
After suspending beads for the final wash, transfer the entire sample to a clean EP tube and then centrifugation.
Washing times are not sufficient. Increase the number of washes.
Increase duration time of washes.
No signal is observed. Flag tagged protein is not expressed in the sample. Make sure the protein of interest contains the FLAG sequence.
Prepare the fresh lysate.
Use appropriate protease inhibitors.
Incubation times are inadequate. Increase the incubation times.
Interfering substance is present in sample. The lysate may contain high concentrations of dithiothreitol (DTT), 2-mercaptoethanol, or other reducing agents.
Excessive detergent concentration may interfere with the antibody-antigen interaction.

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Anti-Flag magnetic beads

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