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Home > qPCR > All-in-One cDNA Synthesis SuperMix

All-in-One cDNA Synthesis SuperMix 

Cat.No. Size Price Quantity  
B24403 200 rxns USD 300
B24408 1000 rxns USD 1000
Free Overnight Delivery on all orders over 500 USD.
Tel: (832) 582-8158Email: [email protected]

Advantages

Reproducibility: Modified Oligo dT: Random primers ratio facilitates qPCR repeatability.
High compatibility: cDNA product is suitable for application with SYBR Green or Probe qPCR.

Customer Reviews

Product Use Citation(32)








Product Comparison

Comparison of cDNA synthesis using Bimake BioScript All-in-One cDNA Synthesis SuperMix and Invitrogen SuperScript® First-Strand Synthesis System for RT-PCR

Total RNA of Hela cells (10-fold gradient) was used for cDNA synthesis. For Real-Time PCR, 1μl cDNA product was used as template using Bimake™ 2x SYBR Green qPCR Master Mix for detection of high-(ACTB), medium-(B2M), and low-expression (TBP) gene, respectively.

These data show that:
Bimake All-in-One presents reliable linear relation within broad range of RNA template concentration and permits high amplification specificity. For amplification efficiency and detection sensitivity, especially at low RNA template concentration, Bimake All-in-One has similar reverse transcription performance compared with Invitrogen SuperScript®.

Price Comparison

Description

BioScript All-in-One cDNA Synthesis SuperMix provides first-strand cDNA synthesis, optimized with the high-temperature capability of Reverse Transcriptase and an optimized SuperMix formula. In one tube, the pre-blended 5× qRT SuperMix contains all the necessary components for reverse transcription of YOUR RNA template. The resulting cDNA product is suitable for use in real-time quantitative RT-PCR (qRT-PCR). Besides, The reverse transcriptase in this kit is purified from E. coli expressing the pol gene of M-MLV, mutagenized to increase thermal stability and cDNA synthesis and to reduce RNase H activity.

Kit Components

Component B24403 B24408
5×qRT SuperMix * 400 μL 1 mL × 2
5 × No RT ControlMix ♥ 40 μL 200 μL
RNase-free Water 1 mL × 2 1mL × 10
* contains Buffer, dNTP, Reverse Transcriptase, RNase inhibitor, Oligo dT/Random primer mix.
♥ contains all the components in 5 × qRT SuperMix, except Reverse Transcriptase.

Application

Synthesis of cDNA from target genes for qRT-PCR, especially low-copy genes or RNA templates of low concentration.

Storage

Store at -20°C for up to 2 years. Repetitive freeze-thaw cycles should be avoided. It is recommended to pre-aliquot the mix into small batches for frequent usage. Product quality is guaranteed under proper storage conditions.

Stability Test

These data showed that, compared to that stored at -20 ℃, after stored at 4 ℃ for 7 days BioScript All-in-One cDNA Synthesis SuperMix showed no significant difference regarding either amplification efficiency or detection sensitivity when used for reverse transcription followed by working with Bimake 2 x SYBR Green qPCR Master Mix.

Caution

For Research Use Only! Not intended for any therapeutic or diagnostic procedures!

File Download

User Guide | BioScript All-in-One cDNA Synthesis SuperMix

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