|Storage buffer||10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.|
|Storage||Store at –20°C.|
Specimen Preparation (forcultured cell lines, IF-IC)
1. Aspirate liquid, then cover cells to a depth of 2–3 mm with 4% formaldehyde diluted in 1X PBS.
1. Add theblocking buffer and incubate for 60 min at RT.
Histone H3 (mono methyl R2) Rabbit Recombinant mAb detects endogenous levels of total Histone H3 (mono methyl R2).
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Histone proteins are highly post-translationally modified. Covalently bonded modifications include acetylation and methylation of the N-terminal tails. These modifications may alter expression of genes located on DNA associated with its parent histone octamer. Histone methylation occurs on arginine, lysine and histidine amino acids residues. Mono-, di- or tri-methylation has been discovered on histone H2A, H3 and H4. Histone methylation has been associated with various cellular functions such as transcription, DNA replication, and DNA damage response including repair, heterochromatin formation, and somatic cell reprogramming. Histone arginine methylation is one such modification that has been linked to transcriptional regulation. Arginines are methylated on the terminal guanidino nitrogens and can exist in three different methylation states; monomethylated (me1), symmetrically dimethylated (me2s) or asymmetrically dimethylated (me2a). H3R2me1 does not inhibit H3K4 methylation. It is present throughout the coding region of genes and correlates with active transcription.