|Reactivity||Human Mouse Rat|
|Storage buffer||10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.|
|Storage||Store at –20°C.|
NOTE: Do not allow slides to dry at any time during this procedure.
1. Deparaffinize/hydrate sections:
1. Incubate sections in three washes of xylene for 5 min each.
2. Wash sections two times in dH2O for 5 min each.
For Citrate: Heat slides in a microwave submersed in 1X citrate unmasking solution until boiling is initiated; continue with 10 min at a sub-boiling temperature (95°-98°C). Cool slides on bench top for 30 min.
1. Wash sections in dH2O three times for 5 min each.
Specimen Preparation (forcultured cell lines, IF-IC)
1. Aspirate liquid, then cover cells to a depth of 2–3 mm with 4% formaldehyde diluted in 1X PBS.
1. Add theblocking buffer and incubate for 60 min at RT.
|Specificity||Histone H2A (acetyl K9) Rabbit Recombinant mAb detects endogenous level of total Histone H2A (acetyl K9).|
|Background||Because of the specific nucleosomal protein-protein and protein-DNA interactions of each of the core histones, they are subject to different degrees of structural constraint probably resulting in different potentials to evolve variants. The H2A family contains a plethora of variants with some "universal variants" found in almost all organisms, namely H2A.Z and H2A.X. various posttranslational modifications are known to occur in histone H2A, e.g., phosphorylation of S1, acetylation of K5, K9 and K13, ubiquitination of K119, phosphorylation T120, and methylation of K125 or K127. It has been proposed that acetylation of K9 in histone H2A might be associated with transcriptionally active chromatin|